Photo by http://benvandenbroecke.be/ Copyright, ISAPP 2019.
High-throughput DNA sequencing technologies and advanced computational techniques of the past few decades have allowed significant advancements in knowledge about human-associated microorganisms. Different areas of the human body—digestive tract (‘gut’), skin, vaginal tract, oral cavity, and others—are home to different communities of microorganisms, called microbiotas. The word microbiome generally refers to the microorganisms in a defined environment (e.g. the human colon) along with the environment itself. While microbiome and microbiota are sometimes used interchangeably, microbiota properly refers to the microorganisms themselves: bacteria, archaea, lower and higher eukaryotes, and viruses in a defined environment. All of these microorganisms comprise the microbiota, even though many “microbiome” studies only focus on the bacterial components.
It is now clear that the human microbiota plays important roles in health and disease.
The gut microbiota
The gut microbiota is the most studied microbiota associated with the human body, with the digestive tract containing the highest density of human-associated microorganisms. Bacteria in the gut are involved in digestion; harvesting energy from food; producing neurotransmitters, enzymes, and vitamins; and furthermore, they participate in immune and metabolic functions and homeostasis.
To date, researchers have been unable to define a ‘healthy gut microbiota’ because the community of gut microbes in a healthy person can look many different ways. Differences in gut microbiota composition have been demonstrated in people with various chronic diseases, compared to healthy controls, but in most cases, causality has not been determined.
Diet and medications are important factors that account for gut microbiota variability in large cohort studies. Antibiotics are a particularly powerful way to alter the gut microbiota: when someone is on antibiotics, the microbial community is altered drastically throughout the course of the medication but microbes gradually return to their baseline levels (or close to baseline levels) after the treatment ends.
Dietary changes may also modulate the gut microbiota in a matter of days; probiotics, prebiotics, fermented foods, and fiber are dietary means of influencing the gut microbiota or its output. Indeed, prebiotics in particular must by definition have a mechanism of action that involves their use by gut microorganisms.